Nanostructures of the Infective Apparatus of Salmonella
Salmonella cause typhoid fever and food poisoning. One of the key structural features of the infection process for this bacterium is the “type III secretion system” (TTSS). This enables it to secrete bacterial proteins into the host cell. The central component of this apparatus has a structure akin to that of a hollow needle, whose length is crucial for the success of the infection process.
Dr. Thomas C. Marlovits, scientific head of the new “Vienna Spot of Excellence”, together with Prof. Jorge E. Galan (Yale University, USA) and other colleagues from the USA, has now explained how the exact length of the needle is determined during the assembly of this biological nano-machine. Says Dr. Marlovits: “A fine example of molecular multi-tasking, the TTSS is not only responsible for transporting bacterial proteins into the host cell, but also for its own assembly from some 200 individual structural proteins. The length of the needle structure is controlled by a sophisticated mechanism. The core of this mechanism is the change in the specificity of the TTSS for different proteins. Although the TTSS still has a high specificity for its own structural proteins during the initial phase of the assembly process, this specificity changes later to handle the proteins that are important for the actual infection process. A change in the structure of the TTSS is crucial for this transformation."
In actual fact, the TTSS comprises four important components: a base anchored into the bacterial membrane with a socket-like structure, plus an overlying inner ring structure on which the needle is built. Marlovits has now succeeded in demonstrating that the ring structure firmly binds the needle with the socket-like structure and the base. This bond also effects a structural change to the base, which impacts on its ability to bind to proteins from the cytoplasmic side of the cell. In this situation, the structural change acts as a signal to indicate that the needle is finished. Instead of assembly proteins, the proteins that are then transported are the ones required for the infection process.
Crucial for the impressive results obtained by Marlovits and his team was the combination of high-resolution imaging methods – cryo electron microscopy - with the molecular genetic analysis of mutants that form unusually long needle structures. The team knew that the protein InvJ influenced needle length - but how this influence is exerted was not fully understood. Marlovits’ comparison yielded a surprisingly clear picture: the mutants lacked the inner ring structure completely. Since these mutants are nevertheless able to form needle structures, and indeed extremely long ones, it was suspected that the inner ring structure provides a type of stop signal for the needle-building process - a signal that the mutants lacked. Further analyses then showed further clear structural differences between the bases of the wild types and those of the mutants. Marlovits’ hypothesis is now that this structural change influences the binding of other proteins that are channelled via the TTSS – and thus provides the stop signal for the needle-building process.
In addition to the basis for further work on the infection channel, this stop signal hypothesis, published in Nature, also represents an excellent starting signal for Marlovits in the work just begun at the “Vienna Spot of Excellence” at the Research Institute of Molecular Pathology (IMP) and the Institute of Molecular Biotechnology (IMBA).
Original publication: Assembly of the inner rod determines needle length in the type III secretion injectisome. NATURE 441, 637-640 (1 June 2006) | doi:10.1038/nature04822
About the “Vienna Spots of Excellence”
The “Vienna Spots of Excellence” are a funding initiative by the City of Vienna. They aim at promoting research projects carried out in cooperation by commercial and scientific institutions. Following the first call earlier this year, three projects were chosen for funding in the program. One of them is located at the “Campus Vienna Biocenter” and is known under the working title of “CMCN – Center of Molecular and Cellular Nanostructure Vienna”. The scientific coordinator of the project is Thomas Marlovits, formerly at the Yale School of Medicine and now a joint Group Leader at the IMP- IMBA Research Center.
About the IMP- IMBA Research Center
The Research Institute of Molecular Pathology (IMP), established in 1988 by Boehringer Ingelheim, and the Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), which went into operation in 2003, have agreed on a close research collaboration. Under the name "IMP-IMBA Research Center", the two institutes share most of the administrative and scientific infrastructure. Together, IMBA and IMP employ over 300 people from 30 different nations. Both institutes are members of the "Campus Vienna Biocenter".
About the Campus Vienna Biocenter
The Campus Vienna Biocenter is one of the largest R&D clusters in Austria with approx. 1,000 scientists from 40 nations at 16 organisations. Academic departments (University of Vienna, Medical University of Vienna, subsumed in the Max F. Perutz Laboratories), private research institutes (IMP - Institute of Molecular Pathology) and institutes of the Austrian Academy of Sciences (IMBA - Institute of Molecular Biotechnology and GMI - Gregor Mendel-Institute) are also located here along with commercial R&D companies, service companies and training course centres (www.viennabiocenter.com).
Supported by Innovatives-oesterreich.at, the agency PR&D, Campus Vienna Biocenter Association and dialoge gentechnik