Content

You are here:

Daniel Gerlich

Cytoskeletal and membrane dynamics in cell division

Dividing cells undergo a dramatic reorganization when they switch from a state supporting cell growth to a state supporting partitioning of their contents. Our laboratory aims to understand the complex interplay between membranes and cytoskeletal structures that mediates chromosome segregation and cytokinesis in human cells. By gaining insight into the underlying molecular mechanisms, we aim to elucidate general principles of dynamic cell organization.  

Our interdisciplinary team of biologists and computer scientists develops technology for automated live-cell microscopy and computer vision to investigate mitosis at a systems level. We combine various fluorescence and electron microscopy techniques into correlative workflows to bridge resolution gaps in the study of transient and small-scale events. These tools and a broad range of biophysical and chemical perturbation approaches enable our laboratory to dissect regulatory networks and mechanisms of cell division.

Contact:

Daniel Gerlich
Senior Scientist
send an email

 

Selected Publications

(2011)
 
Guizetti, J., Schermelleh, L., Mäntler, J., Maar, S., Poser, I., Leonhardt, H., Müller-Reichert, T., Gerlich, DW. (2011). Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science. 331(6024):1616-20 (abstract)
 
(2010)
 
Held, M., Schmitz, MH., Fischer, B., Walter, T., Neumann, B., Olma, MH., Peter, M., Ellenberg, J., Gerlich, DW. (2010). CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Nat Methods. 7(9):747-54 (abstract)
 
Schmitz, MH., Held, M., Janssens, V., Hutchins, JR., Hudecz, O., Ivanova, E., Goris, J., Trinkle-Mulcahy, L., Lamond, AI., Poser, I., Hyman, AA., Mechtler, K., Peters, JM., Gerlich, DW. (2010). Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells. Nat Cell Biol. 12(9):886-93 (abstract)
 
(2009)
 
Steigemann, P., Wurzenberger, C., Schmitz, MH., Held, M., Guizetti, J., Maar, S., Gerlich, DW. (2009). Aurora B-mediated abscission checkpoint protects against tetraploidization. Cell. 136(3):473-84 (abstract)