Nol9 is a novel polynucleotide 5'-kinase involved in ribosomal RNA processing

November 09, 2010

Depletion of human Nol9 impairs processing of the 28S ribosomal RNA. Metabolic labeling of HeLa cells transfected with siRNAs targeting a control gene or Nol9. Cells were incubated with 3H-methyl-methionine and chased in non-radioactive medium for the ind

Ribosomes are large ribonucleoproteins that translate the genetic information from RNA into protein. Therefore, protein production is fully dependent on the sufficient availability of the components of ribosomes, ribosomal proteins and ribosomal RNAs (rRNAs). We have identified a novel RNA 5’-kinase, Nol9, which is required for efficient biogenesis of the large ribosomal subunit RNAs, 5.8S and 28S rRNAs, in human cells. Depletion of Nol9 leads to a severe decrease of the 60S large ribosomal subunit and consequently the mature ribosome.

>>link to article on EMBO Journal website

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